Methods: Based on classic Berger's primary cell culture method, We used low conceptus age rat embryos, and add the fetal bovine serum into the culture solution for the first time, to increase the dopaminergic neuron adherence and growth; 方法:以Nakai等经典神经元细胞原代培养方法为基础,通过使用低日龄胎鼠,初次培养液加入胎牛血清等步骤,促进中脑多巴胺能神经元细胞贴壁和生长;